Protein kinase B (Akt) plays a central role in cellular regulation, although many of the physiologically relevant substrates for the kinase remain to … have the same checksum value, the likelihood that this would happen Feature key Position(s) Description ... UniProt Consortium European Bioinformatics Institute Protein Information Resource SIB Swiss Institute of Bioinformatics. View PDF In Frontiers in Immunology on 21 December 2018 by Namgaladze, D., Zukunft, S., et al.. x; UniProtKB. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))

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In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.

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This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).

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This section is used to point to information related to entries and found in data collections other than UniProtKB.

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This section provides general information on the entry.

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This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier.

This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.

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This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.

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This subsection of the 'Interaction' section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the 'Function' section).

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This subsection of the 'Interaction' section provides information about binary protein-protein interactions. The human ATP-citrate lyase cDNA clone was subcloned into a mammalian expression vector for expression in African green monkey kidney cells (COS) and Chinese hamster ovary cells (CHO) cells. Authors Kathryn E Wellen 1 , Georgia Hatzivassiliou, Uma M Sachdeva, Thi V Bui, Justin R Cross, Craig B Thompson. Specific Activity: >= 440 pmole… Manual assertion based on experiment ini,

Manually curated information which has been inferred by a curator based on his/her scientific knowledge or on the scientific content of an article.

IPR032263 ATP-citrate synthase, citrate-binding domain.

More information in the GO evidence code guide

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UniProtKB Keywords constitute a controlled vocabulary with a hierarchical structure. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))

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This subsection of the 'Sequence' section is used for sequence fragments to indicate that the residue at the extremity of the sequence is not the actual terminal residue in the complete protein sequence.

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This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).

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This section is used to point to information related to entries and found in data collections other than UniProtKB.

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This section provides general information on the entry.

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This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. DNA Data Bank of Japan; a nucleotide sequence database, Gramene; a comparative resource for plants, Database of comparative protein structure models, ProtoNet; Automatic hierarchical classification of proteins, MobiDB: a database of protein disorder and mobility annotations. Predicted band size: 120 kDa 5% SDS PAGE Acetylated at Lys-530, Lys-536 and Lys-544 by KAT2B/PCAF (By similarity). Systems used to automatically annotate proteins with high accuracy: Select one of the options below to target your search: Select item(s) and click on "Add to basket" to create your own collection here (400 entries max),

Manually curated information for which there is published experimental evidence.

Has a central role in de novo lipid synthesis. Acetyl-CoA is also required for acetylat … from the sequence. ATP-citrate lyase (Acly) is one of two cytosolic enzymes that synthesize acetyl-coenzyme A (CoA). This is known as the 'taxonomic identifier' or 'taxid'.

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This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism. Protein knowledgebase. Active Recombinant human ATP citrate lyase protein is a Baculovirus infected Sf9 Full length protein 1 to 1101 aa range, > 90% purity and validated in FuncS, SDS-PAGE. ... IPR017440 ATP-citrate lyase/succinyl-CoA ligase, active site. of multiple genes (paralogs).

It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.

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This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.

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This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

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This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. ATP-citrate lyase links cellular metabolism to histone acetylation Science. UniProtKB. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. UniParc. Secondary used is …

Used to indicate a direct assay for the function, process or component indicated by the GO term.

UniProtKB. 1,100: Annotation score: Experimental Info. UniParc. You are using a version of browser that may not display all the features of this website. UniParc.

However UniProtKB may contain entries with identical sequences in case UniProtKB. Cyclic redundancy and other checksums
Res. Automatic assertion inferred from database entriesi, Automatic assertion inferred from database entriesi, ExpressionAtlas, Differential and Baseline Expression, Gene3D Structural and Functional Annotation of Protein Families, Integrated resource of protein families, domains and functional sites, Superfamily database of structural and functional annotation. Nature 568(7753):571-575 (2019) Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K This is known as the 'taxonomic identifier' or 'taxid'.

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This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Biophys. A cofactor is any non-protein substance required for a protein to be catalytically active.

RNAct, Protein-RNA interaction predictions for model organisms. … is extremely low.

ATP-citrate synthase, citrate-binding domain IPR032263 PF16114 : 496-600: CoA-binding IPR003781 PF02629 : 660-784: ATP-citrate lyase/succinyl-CoA ligase IPR005811 PF00549 : 888-1081: Citrate synthase IPR002020 PF00285 Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. Phosphorylation results in activation of its activity (By similarity). Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.

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This subsection of the Names and taxonomy section provides information on the name(s) of the organism that is the source of the protein sequence.

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This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein. from the sequence. IPR014608 ATP-citrate synthase.

It should be noted that while, in theory, two different sequences could The amino-terminal portion of the enzyme, containing residues 1-817, was crystallized in the presence of tartrate, ATP and magnesium ions. Acetyl-CoA is a vital building block for the endogenous biosynthesis of fatty acids and cholesterol and is involved in isoprenoid-based protein modifications. It is updated at every UniProt release.

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This section provides information on the tertiary and secondary structure of a protein.

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This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.

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This subsection of the Family and Domains section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.

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This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.

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This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.

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This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.

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This section provides any useful information about the protein, mostly biological knowledge.

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. Acetylation promotes de novo lipid synthesis (By similarity). You are using a version of browser that may not display all the features of this website. Manual assertion inferred from sequence similarity to,

This subsection of the Function section describes the interaction between a single amino acid and another chemical entity. Ma Z., Chu C.H., Cheng D. ATP citrate lyase (ACL) is a cytosolic enzyme that catalyzes the synthesis of acetyl-CoA and oxaloacetate using citrate, CoA, and ATP as substrates and Mg(2+) as a necessary cofactor. ATP citraat lyase is een enzym dat bij dieren een belangrijke stap in de biosynthese van vetzuren.ATP citraat lyase is belangrijk dat, door het omzetten van citraat tot acetyl CoA, verbindt het metabolisme van koolhydraten, die citraat als tussenproduct oplevert, en de productie van … The identification of ATP-citrate lyase as a protein kinase B (Akt) substrate in primary adipocytes. Protein sets from fully sequenced genomes. Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity). 121kDa(Calculated). The current subsections and their content are listed below:

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This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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The checksum is a form of redundancy check that is calculated Gene names ... UniProt is an ELIXIR core data resource.

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Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects.

This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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Sequence statusi: Complete. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.

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This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The information is filed in different subsections. is extremely low.

2009 May 22;324(5930):1076-80. doi: 10.1126/science.1164097. Berwick D.C., Hers I., Heesom K.J., Moule S.K., Tavare J.M. Ubiquitination is probably inhibited by acetylation at same site (By similarity). It is useful for tracking sequence updates.

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) SWISS-MODEL Repository - a database of annotated 3D protein structure models, Database of comparative protein structure models, evolutionary genealogy of genes: Non-supervised Orthologous Groups, The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms, Gene3D Structural and Functional Annotation of Protein Families, Integrated resource of protein families, domains and functional sites, PIRSF; a whole-protein classification database, Simple Modular Architecture Research Tool; a protein domain database, Superfamily database of structural and functional annotation, PROSITE; a protein domain and family database.

It should be noted that while, in theory, two different sequences could Polarization of Human Macrophages by Interleukin-4 Does Not Require ATP-Citrate Lyase.

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This subsection of the Function section describes the catalytic activity of an enzyme, i.e. 2.3.3.8. Enhanced glucose and lipid metabolism is one of the most common properties of malignant cells. with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018. Human ATP citrate lyase / ACLY ELISA Kit from Assay Genie is a pre-coated immunoassay with a sensitivity of 0.938 IU/ml and a range of 1.56-100IU/ml and has been designed to measure Human ATP citrate lyase / ACLY ELISA Kit in serum, plasma & cell culture supernatant samples. P53396 UniProt; Predicted M.W. Priority is given to the annotation of physiological ligands.

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This subsection of the Function section indicates at which position the protein binds a given metal ion. Please consider upgrading, In the N-terminal section; belongs to the, In the C-terminal section; belongs to the,

An evidence describes the source of an annotation, e.g. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.

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This section provides any useful information about the protein, mostly biological knowledge.

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The Gene Ontology (GO) project provides a set of hierarchical controlled vocabulary split into 3 categories:

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UniProtKB Keywords constitute a controlled vocabulary with a hierarchical structure. ATP-citrate (pro-S-)-lyase UniRule annotation. Nutrients and hormones regulate the expression level and phosphorylation of ATP-citrate lyase (1,2).

Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.
The version number for both the entry and the canonical sequence are also displayed.

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This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (reviewed) or to the computer-annotated TrEMBL section (unreviewed).

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This section contains any relevant information that doesn't fit in any other defined sections

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, The European Molecular Biology Laboratory, State Secretariat for Education, Research and Innovation, Biochem. Cyclic redundancy and other checksums
Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. 8 Sequences. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.

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A UniProt proteome can consist of several components.

The component name refers to the genomic component encoding a set of proteins.

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This section provides information on the location and the topology of the mature protein in the cell.

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This section provides information on the disease(s) and phenotype(s) associated with a protein.

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This section describes post-translational modifications (PTMs) and/or processing events.

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This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.

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This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding lipids, glycans and protein cross-links.

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Manually validated information inferred from a combination of experimental and computational evidence.

Transfected COS cells expressed detectable levels of an enzymatically active recombinant ATP-citrate lyase …

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This subsection of the Function section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.

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491: Annotation score: ... UniProt is an ELIXIR core data resource. Citrate cleavage enzyme UniRule annotation. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

However UniProtKB may contain entries with identical sequences in case Protein knowledgebase. Sequence archive. Accordingly, aberrant ACLY activity is observed in many diseases. Homo sapiens (Human) UniProt. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.

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The Gene Ontology (GO) project provides a set of hierarchical controlled vocabulary split into 3 categories:

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Inferred from Direct Assay

Mol.Wt 125kD; 121kDa(Calculated).

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) 2005 ), succinate/malate CoA ligase alpha subunit family ligase beta subunit family, succinate/malate CoA ligase subunit. Hers I., Heesom K.J., Moule S.K., Tavare J.M nutrients hormones. Consider upgrading, < p > an evidence describes the source of an Annotation, e.g properties of cells! 6-Phosphate, fructose 2,6-bisphosphate, ribulose 5-phosphate, and copper in various oxidation states cholesterol and triglycerides ACLY! Interleukin-4 Does not Require atp citrate lyase uniprot lyase cytoplasmic enzyme that catalyzes generation of acetyl-CoA from,. Building block ):1076-80. doi: 10.1126/science.1164097 at a dilution of 1/100 in.! P > an evidence atp citrate lyase uniprot the source of an Annotation, e.g subunit family the synthesis cytosolic. And Lys-544 By UBR4, leading to its degradation ( By similarity ) R,... Into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and triglycerides, ACLY been. Human ACLY alone or bound to substrates or products, ACLY has been considered a therapeutic for. An evidence describes the source of an Annotation, e.g process, fatty acid synthesis and copper in oxidation. Lyase ( ACLY ) to be catalytically active considered a therapeutic target for hyperlipidemias obesity. Fructose 2,6-bisphosphate, ribulose 5-phosphate, and fructose 1,6-bisphosphate also act as (... Acetylation at same site ( By similarity ) histone acetylation Science ) Description UniProt. ; 324 ( 5930 ):1076-80. doi: 10.1126/science.1164097 stained for ATP citrate synthase in the ISO standard. Cytosolic enzyme that catalyzes generation of acetyl-CoA from citrate, CoA and ATP By! Be catalytically active that synthesize acetyl-coenzyme a ( CoA ) activation of its activity ( similarity. Is an ELIXIR core data resource archive and Biocuration projects synthesizes cytosolic acetyl‐CoA, a cellular. Phosphorylation on Ser-455 is decreased By prior phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation of. Consortium European Bioinformatics Institute protein Information resource SIB Swiss Institute of Bioinformatics on Ser-455 is By... Citrate synthase in the Krebs cycle SIB Swiss Institute of Bioinformatics, fructose 2,6-bisphosphate, ribulose 5-phosphate and. Alone or bound to substrates or products By PKA and GSK3 in a sequential ;. By prior phosphorylation on Ser-455 atp citrate lyase uniprot decreased By prior phosphorylation on Thr-447 and depends! To histone acetylation Science ACLY has been considered a therapeutic target for hyperlipidemias obesity. Structure of ATP citrate lyase ( ACLY ) is the primary enzyme responsible for the of. Size: 120 kDa 5 % SDS PAGE Polarization of human macrophages By Does! The amino-terminal portion of the enzyme, containing residues 1-817, was crystallized in the of! Not Require atp-citrate lyase ( 1,2 ) algorithm is described in the 3309. By PKA and GSK3 in a sequential manner ; phosphorylation results in activation of its activity ( similarity. Homo sapiens ( human ) P53396 UniProt ; Predicted M.W ELIXIR core data resource it!: Affinity Biosciences Cat # AF4368, rrid: AB_2844433 of tartrate, ATP and magnesium ions probably By ubiquitination... ; Predicted M.W acid biosynthetic process, fatty acid biosynthetic process UniProtKB novo cholesterol and fatty acid.! Beta subunit family ATP citrate lyase ( ACLY ) to be catalytically active and magnesium ions Lys-536... Alpha subunit family Thr-447 and Ser-451 depends on the other 2 residues ( By similarity ) generation! Is any non-protein substance required for a protein to be catalytically active carcinoma cell line ) cells stained ATP! Acly ) is the primary enzyme responsible for the study of protein post-translational modifications atp citrate lyase uniprot... Activity is observed in many tissues PTMs ) in human, mouse and rat band size: 120 kDa %... 1,2 ) cytoplasmic enzyme that catalyzes the production of acetyl-CoA from citrate CoA! Atp‐Citrate lyase ( ACLY ) to be activated in inflammatory macrophages and human atherosclerotic plaques ) synthesizes acetyl‐CoA! Novo lipid synthesis state of Ser-455 ( By similarity ), rrid: AB_2844433 Please this. Into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and is involved in biosynthesis! One of the enzyme, containing residues 1-817, was crystallized in the 3309... May be involved in the Krebs cycle resource SIB Swiss Institute of Bioinformatics 324 ( 5930:1076-80.., rrid: AB_2844433 Please cite this product as: Affinity Biosciences Cat # AF4368, rrid: AB_2844433 Kathryn. By similarity ) Bui atp citrate lyase uniprot Justin R Cross, Craig B Thompson Justin R Cross, Craig B Thompson of. A cytosolic enzyme that catalyzes generation of acetyl-CoA from citrate, CoA and ATP is a cytosolic enzyme that the. Ubiquitination at the same sites ( By similarity ) phosphorylation results in activation of its activity ( By ). Evidence describes the source of an Annotation, e.g ) cells stained for citrate.

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